FastQCFastQC Report
Mon 6 Jun 2016
ERR1378683.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378683.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159636
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCATGATCTCGTATGCC12150.10477425674953174Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAG250.002101413436.0198826
GACCGTT1250.032.39692729
AGACCGT1450.029.4799628
CGTATGC1400.028.92707444
TCTCGTA1400.028.92707441
TCGTATG1400.028.92582743
ACCGTTC1450.027.92838330
CGTTCAT1550.027.57921832
CCGTTCA1550.027.57921831
CGGTTCA1500.026.9962759
CTCGTAT1550.026.1276842
GCGGTTC1550.026.125438
GAGACCG1600.025.31009927
AGCGGTT1650.024.5420677
GAGCGGT1750.023.155646
ATCTCGT1750.023.1416640
AGAGCGG1800.022.5124245
GATCGGA602.469833E-422.49689718
ATGCCGA1900.022.49689522
CCGAGAC1850.021.88981425
Produced by FastQC (version 0.11.5)