FastQCFastQC Report
Mon 6 Jun 2016
ERR1378681.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378681.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519324
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCATTATCTCGTATGCC10060.19371336583712673Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCAT1450.040.34068332
TCGTATG1550.039.18952643
CTCGTAT1550.037.73806442
CGTATGC1650.036.81440444
ATCTCGT1700.035.73162540
GCGGTTC1850.035.2666478
CCGTGCA1600.035.1526431
GACCGTG1700.034.40823429
TATCTCG1700.034.40823439
TCTCGTA1700.034.40823441
AGACCGT1700.034.40823428
GAGCGGT1900.034.3617366
AGAGCGG1950.033.4806675
AGCGGTT1950.033.45817
GTATGCC1900.031.970445
CGGAAGA2050.031.8536011
CCGAGAC1850.031.61837425
AAGAGCG2100.031.089194
GTTCAGC2100.031.06823711
GAGACCG1900.030.78631227
Produced by FastQC (version 0.11.5)