FastQCFastQC Report
Mon 6 Jun 2016
ERR1378676.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378676.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975171
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG650.041.53756332
CCGTTAC751.8189894E-1235.9992231
CGTATGC803.6379788E-1233.74926844
ACCGTTA803.6379788E-1233.74926830
AGACCGT855.456968E-1231.76401928
GACCGTT901.2732926E-1129.99935229
GCGGTTC901.2732926E-1129.9978128
CCGACGG300.005148716329.99781217
CCGAGAC1053.6379788E-1227.85653925
GAGCGGT1004.5474735E-1127.006346
CTCGTAT1004.5474735E-1126.99941642
TCGTATG1004.5474735E-1126.99941643
GTTACGT1058.54925E-1125.7137333
AATGCCG1058.54925E-1125.7124121
TATCTCG1101.5097612E-1024.54492239
TCTCGTA1101.5097612E-1024.54492241
AGCGGTT1101.5279511E-1024.5436637
CGTATCT1201.6370905E-1124.37447437
ATCTCGT1152.6557245E-1023.47775340
CGGTTCA1252.910383E-1123.3982939
Produced by FastQC (version 0.11.5)