FastQCFastQC Report
Mon 6 Jun 2016
ERR1378673.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378673.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188278
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATA1100.038.86098530
CGATATC1100.038.86098532
GACCGAT1150.037.17137529
CTCGTAT1200.035.62256642
CCGATAT1200.035.62256631
TCTCGTA1250.034.19766641
TCGTATG1250.034.19766643
CATCTCG1350.033.3310639
AGAGCGG1300.032.89765
CGTATGC1350.031.66450544
ATGCCGA1450.031.03236422
GAGCGGT1400.030.547776
AATGCCG1500.029.99795321
AAGAGCG1550.029.0437184
GCGGTTC1500.028.4980558
AGCGGTT1500.028.4980557
ATCTCGT1600.026.71692740
CGGTTCA1650.025.9073249
GAGACCG1650.025.90732427
AGACCGA1700.025.14534228
Produced by FastQC (version 0.11.5)