FastQCFastQC Report
Mon 6 Jun 2016
ERR1378669.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378669.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283990
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAC403.460773E-739.371932
GAGCGGT606.3973857E-933.76976
CTCGTAT554.1679905E-628.63410842
CCGCTTA608.197536E-626.24793431
GCGGTTC608.200015E-626.246918
AGCGGTT608.200015E-626.246917
TCTCGTA702.7049571E-522.49822841
AGACCGC802.9772382E-622.49822828
ACGCCCG702.7057711E-522.4973512
CGCCCGG1257.530616E-1021.5974613
CGTATGC807.568456E-519.6859544
TCGTATG807.568456E-519.6859543
CGGTTCA851.2056676E-418.5272319
GACCGCT1053.1149844E-517.14150829
AGAGCGG952.8071564E-416.5886235
ATCTCGT952.8213728E-416.5776440
GCTCCGC952.8213728E-416.5776436
AAGAGCG1004.149053E-415.7591934
ACCGCTT1156.788053E-515.65094130
ATGCCGA1209.763359E-514.99823422
Produced by FastQC (version 0.11.5)