FastQCFastQC Report
Mon 6 Jun 2016
ERR1378667.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378667.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124463
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG653.6743586E-1034.61230539
GACCGTT708.1490725E-1032.1429
CGTATGC751.7043931E-929.99733444
CCGTTGA751.7043931E-929.99733431
CTCGTAT803.3978722E-928.12249842
TCGTATG803.3978722E-928.12249843
ACCGTTG803.3978722E-928.12249830
AGACCGT957.421477E-1026.05031628
CGTTGAC1052.3683242E-923.56933232
TCTCGTA1056.1132596E-821.42666441
GTATGCC1403.090463E-919.28399845
AAGAGCG1102.2453642E-618.41814
GAGCGGT1102.2463191E-618.417286
AGAGCGG1102.2463191E-618.417285
CGGTTCA1507.2723196E-917.99849
GCGGTTC1205.1772786E-616.87358
GAGACCG1205.1772786E-616.873527
ATGCCGA1205.1772786E-616.873522
ATCTCGT1401.2558012E-616.0740
TCGTATA850.002566640515.8809410
Produced by FastQC (version 0.11.5)