FastQCFastQC Report
Mon 6 Jun 2016
ERR1378665.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378665.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360904
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCA1550.031.93238330
CTCGTAT1500.031.49925642
TCGTATG1500.031.49809843
GAGCGGT1650.031.3790366
AGACCGT1600.030.93449428
CCGTCAA1650.029.9992931
CGTCAAC1650.029.9992932
GACCGTC1650.029.99708629
TGCCGAG1650.029.99708623
TCTCGTA1600.029.53055241
CGTATGC1650.028.63568544
TATCTCG1650.028.63568539
AGCGGTT1850.027.9692277
AATGCCG1800.027.49631921
ATGCCGA1850.026.75317422
CCGAGAC2050.026.33890325
GCGGTTC1900.026.049148
GAGACCG2000.024.74759327
CGGTTCA2050.024.1431069
AAGAGCG2150.024.0815854
Produced by FastQC (version 0.11.5)