FastQCFastQC Report
Mon 6 Jun 2016
ERR1378664.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378664.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073781
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTTACATCTCGTATGCC12890.12004310003622713Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG2350.039.2547529
CGTATGC2400.038.43694344
GCGGTTC2450.037.649018
GAGCGGT2500.036.91156
ATCTCGT2500.036.89946440
CTCGTAT2550.036.17594542
AGACCGT2550.036.17594528
TCGTATG2550.036.17594543
TCTCGTA2600.035.48025541
TGCCGAG2600.035.48025523
GAGACCG2550.035.29360627
AGAGCGG2650.034.822175
AATGCCG2650.034.80757521
CGGTTCA2750.034.3599369
ATGCCGA2700.034.1629922
CCGAGAC2800.032.9459525
AAGAGCG2900.031.8202554
AGCGGTT2900.031.8069237
CGGAAGA3000.031.5156881
ACCGTGT2900.031.03403530
Produced by FastQC (version 0.11.5)