FastQCFastQC Report
Mon 6 Jun 2016
ERR1378663.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378663.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238915
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTT650.041.53663331
CGCGTTA650.041.53663332
ACCGCGT751.8189894E-1235.99696430
GCGTTAT803.6379788E-1233.74851633
CTCGTAT901.2732926E-1129.99868442
CGTTATA952.3646862E-1128.41980634
TCTCGTA1058.54925E-1125.71315841
GAGCGGT1159.094947E-1225.4439356
CGTATGC1159.094947E-1225.43366644
TCGTATG1101.5279511E-1024.54437643
GCGGTTC1101.5279511E-1024.5433858
AGCGGTT1101.5279511E-1024.5433857
AGACCGC1201.6370905E-1124.37294628
CGGTTCA1204.5292836E-1022.4981029
ATCTCGT1358.367351E-1121.66571440
GACCGCG1257.530616E-1021.5981829
CCGAGAC1257.530616E-1021.5981825
GAGACCG1401.364242E-1020.89109627
AATGCCG1301.2296368E-920.76747921
AAGAGCG1351.9554136E-920.0071964
Produced by FastQC (version 0.11.5)