FastQCFastQC Report
Mon 6 Jun 2016
ERR1378662.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378662.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051563
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1100.036.81783344
TCGTATG1150.035.21705643
CTCGTAT1250.032.39969342
CGGTTCA1250.032.396619
AGACCGG1350.031.6648628
GACCGGC1300.031.15206729
ATCTCGT1400.030.53542340
CGGCTTA1350.029.99971632
CGGAGCG300.005147301629.99971440
AGCGGTT1350.029.996867
TCTCGTA1450.029.48247741
GAGCGGT1550.029.040276
AAGAGCG1450.027.938744
ACCGGCT1450.027.9294430
TATCTCG1500.026.99974339
GCGGTTC1500.026.9971758
AGAGCGG1550.026.1362445
GAGACCG1700.025.14442827
TGCCGAG1700.025.14442823
ATGCCGA1700.023.82103722
Produced by FastQC (version 0.11.5)