FastQCFastQC Report
Mon 6 Jun 2016
ERR1378660.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378660.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569759
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTG1150.035.21422630
TCTCGTA1350.034.9979741
CGTTGTA1250.034.19801332
CTCGTAT1400.033.7480442
CGTATGC1400.032.1409944
CCGTTGT1350.029.9982631
GACCGTT1350.029.99730529
TCGTATG1700.027.7916243
GAGCGGT1800.025.0120946
AGACCGT1650.024.54324728
GAGACCG1750.024.42637427
CCGAGAC1750.024.42637425
AGCGGTT1900.023.6813287
CGGTTCA2100.023.5685589
CGGAAGA2100.022.5194971
AGAGCGG2150.021.9873755
GCGGTTC2050.021.9485478
AAGAGCG2100.021.4389364
ATCTCGT2250.019.99883840
CATCTCG2400.019.68635739
Produced by FastQC (version 0.11.5)