FastQCFastQC Report
Mon 6 Jun 2016
ERR1378659.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378659.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170081
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1250.034.2004644
ACCGCTC1400.032.1419130
AGACCGC1400.032.1419128
TCTCGTA1350.031.6670941
CGCTCTA1450.031.03489532
CTCGTAT1450.029.4831542
GACCGCT1600.028.12417229
CCGCTCT1650.027.2730931
GCGGTTC1750.026.9968998
TCGTATG1700.026.46980743
AGCGGTT1900.024.8655627
ATCTCGT1750.024.42889640
CCGAGAC1850.024.32360825
GAGCGGT1950.024.2373076
AGAGCGG2050.024.1528575
GAGACCG2050.024.1456327
CGGAAGA1900.023.694651
AAGAGCG2050.023.0549984
CGGTTCA2250.021.9974739
AATGCCG2200.021.47572121
Produced by FastQC (version 0.11.5)