FastQCFastQC Report
Mon 6 Jun 2016
ERR1378657.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378657.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289930
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCATA750.038.9972832
CTCGTAT900.034.99755542
TCGTATG900.034.99755543
GAGCGGT950.033.1697276
CGTATGC1050.032.14061744
TCTCGTA1050.029.99790841
ACCGCCA1001.8189894E-1229.24796130
AGCGGTT1200.028.1230377
AGAGCGG1150.027.4010775
GACCGCC1105.456968E-1226.58905429
TATCTCG1200.026.24816939
AGACCGC1200.026.24816928
CGGTTCA1251.8189894E-1225.1982429
CGGAAGA1301.8189894E-1224.2497581
GCGGTTC1301.8189894E-1224.2290788
AATGCCG1301.8189894E-1224.22907821
AAGAGCG1500.022.5080264
ATGCCGA1459.094947E-1221.72262222
ATCTCGT1653.6379788E-1220.4531240
GAGACCG1703.6379788E-1219.85155727
Produced by FastQC (version 0.11.5)