FastQCFastQC Report
Mon 6 Jun 2016
ERR1378656.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378656.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923350
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG850.037.05962839
ACCGCTT850.037.05561430
CGTATGC950.033.1550244
GACCGCT950.033.1550229
AGACCGC950.033.1550228
CTCGTAT1050.032.1400742
CGGAAGA1150.031.3236851
AGAGCGG1150.031.3168985
AGCGGTT1150.031.3016347
TCTCGTA1100.028.63388341
GAGCGGT1300.027.7034136
GCGGTTC1300.027.6899098
AATGCCG1450.026.37702621
GAGACCG1200.026.24772527
TCGTATG1350.024.99783343
CCGCTTG1301.8189894E-1224.22867231
ATGCCGA1500.023.99792122
TGCCGAG1500.023.99792123
CCGAGAC1353.6379788E-1223.33131225
CGGTTCA1600.022.4980499
Produced by FastQC (version 0.11.5)