FastQCFastQC Report
Mon 6 Jun 2016
ERR1378655.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378655.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081228
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG800.039.37338343
GCGGATT250.002107956735.99685718
AATGCCG1000.031.4972521
GCGGTTC950.030.7867858
CGTATGC1001.8189894E-1229.2501544
GACCGCT1001.8189894E-1229.24744629
AGAGCGG1053.6379788E-1227.8663085
TCTCGTA1053.6379788E-1227.85599941
ACCGCTT1053.6379788E-1227.85471230
AGCGGTT1150.027.3889147
CTCGTAT1105.456968E-1226.58981742
AGACCGC1105.456968E-1226.58858728
GAGCGGT1200.026.2586386
CCGAGAC1201.6370905E-1124.37287125
ATGCCGA1201.6370905E-1124.37287122
CGGTTCA1252.910383E-1123.3979559
AAGAGCG1305.0931703E-1122.5074044
CGCTTCA1305.0931703E-1122.49907532
CGGAAGA1257.4578566E-1021.6171071
ATCTCGT1552.3646862E-1120.32174740
Produced by FastQC (version 0.11.5)