FastQCFastQC Report
Mon 6 Jun 2016
ERR1378654.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378654.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023623
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2000.032.6240942
CCGCCTA2150.031.3944731
AGACCGC2250.030.99913228
CGCCTAA2200.030.6809632
GCGGTTC2300.030.322998
AGCGGTT2300.030.322997
TCTCGTA2200.029.6582641
TCGTATG2200.029.6582643
CGTATGC2400.029.06168644
TATCTCG2250.028.99918739
GACCGCC2350.028.72259729
GAGCGGT2450.028.4770366
ACCGCCT2550.027.35217730
CGGTTCA2550.027.3501479
CCGAGAC2650.025.47035625
AGAGCGG2750.025.370455
ATGCCGA2850.025.26120222
AATGCCG2850.024.47178821
CGGAAGA2750.023.7436221
ATCTCGT2750.023.72660840
Produced by FastQC (version 0.11.5)