FastQCFastQC Report
Mon 6 Jun 2016
ERR1378653.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378653.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492414
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA300.005148588729.99899735
AGGTTCG300.00515027629.99698414
CGTATGC1100.028.63636444
TCTCGTA1150.027.39130241
GAGCGGT1250.027.009056
TCGTATG1105.456968E-1226.58912843
ACCGAAG1251.8189894E-1225.19831330
GCGGTTC1251.8189894E-1225.1974688
CGAAGTT1350.024.99916332
AATGCCG1350.024.99748821
CTCGTAT1201.6370905E-1124.37499842
GACCGAA1301.8189894E-1224.22914529
ATCTCGT1450.023.27586440
AGAGCGG1600.022.507545
CCGAAGT1405.456968E-1222.49924531
CCGAGAC1405.456968E-1222.49849325
AGCGGTT1551.8189894E-1221.7720057
AAGAGCG1651.8189894E-1220.4614014
GAGACCG1603.8198777E-1119.68618227
ATGCCGA1603.8198777E-1119.68552222
Produced by FastQC (version 0.11.5)