FastQCFastQC Report
Mon 6 Jun 2016
ERR1378652.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378652.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542730
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAG950.037.89285331
GACCGTA1050.034.2840129
ACCGTAA1100.032.72564330
CGTATGC1100.032.72458344
GAGCGGT1350.028.3438686
AGACCGT1300.027.6909328
TCTCGTA1300.027.69003341
TCGTATG1300.027.69003343
CTCGTAT1350.026.66447842
CGGTTCA1400.025.7121739
AGAGCGG1600.025.321915
GCGGTTC1450.024.8255488
ATCTCGT1500.023.99880640
CGTAAGT1600.022.4988832
AGCGGTT1600.022.4981527
CCGAGAC1650.021.81709925
ATGCCGA1653.6379788E-1220.4535322
AAGAGCG1950.019.6226794
CGGAAGA2200.019.4453471
AATGCCG1801.0913936E-1118.74906721
Produced by FastQC (version 0.11.5)