FastQCFastQC Report
Mon 6 Jun 2016
ERR1378650.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378650.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737570
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGT1450.043.44694529
CCGGTAA1550.040.6450931
ACCGGTA1700.037.05768630
CGGTAAT1800.034.9999332
CGTATGC2050.034.02432644
TCGTATG1950.033.4605143
AGACCGG2000.031.49903328
TCTCGTA2200.029.65903341
AATGCCG2300.029.34523821
ATGCCGA2400.028.12251922
CTCGTAT2350.027.76590342
GAGACCG2350.027.76430727
GAGCGGT2450.026.6387336
AGCGGTT2600.025.95857
AAGAGCG2700.025.006434
TGCCGAG2700.024.99851423
ATCTCGT2750.024.54540640
CCGAGAC2750.024.54399525
GCGGTTC2800.023.3008468
CGGTTCA2950.022.1160569
Produced by FastQC (version 0.11.5)