FastQCFastQC Report
Mon 6 Jun 2016
ERR1378649.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378649.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178248
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT1159.094947E-1225.43340915
TAAGCCG1301.8189894E-1224.22945813
GATCGCG751.694525E-624.0069664
TGACGTC1252.910383E-1123.39873321
ATCGCGC802.969291E-622.5065335
CGGTATT1003.650166E-822.49878324
CGTCATA1358.367351E-1121.66549524
TTCGGTA1056.117625E-821.42741222
CCGATGA1750.020.57031417
GCCGATG1452.2009772E-1020.17132416
GCGGTCT1151.5978912E-719.5641636
GGCGGTC1151.5978912E-719.5641635
GACGTCA1503.45608E-1019.49894522
AATTCGG1302.777415E-819.03743220
TCGCGCC951.3134231E-518.9528716
ACGTCAT1555.347829E-1018.86994723
TCGGTAT1202.5006375E-718.74898723
CGATGAC1851.6370905E-1118.24225618
AGGCGGT1305.791735E-717.30675534
ATGACGT1908.014467E-915.39390520
Produced by FastQC (version 0.11.5)