FastQCFastQC Report
Mon 6 Jun 2016
ERR1378648.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378648.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094442
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA33190.3032595605797292No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA26040.23792946542621718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG3150.042.85348513
CGGTATT3850.040.9074724
CGTCATA3350.040.2969124
TGACGTC3400.039.70249621
GGCGGTC4150.038.4924535
AATTCGG4150.037.94856620
TCGGTAT4200.037.49680323
AAGCCGA3600.037.496814
GCGGTCT4250.037.05735436
ATGACGT3650.036.98314320
ACGTCAT3650.036.98314323
CGATGAC3650.036.98314318
TCGGGGA4350.036.72100414
ATTCGGT4300.036.62478321
AGCCGAT3750.036.59687815
CTCGGGG3750.036.59687813
AGGCGGT4500.036.49855434
TTCGGTA4350.036.20380822
GACGTCA3800.035.52328522
CGGGGAA4500.035.49697515
Produced by FastQC (version 0.11.5)