FastQCFastQC Report
Mon 6 Jun 2016
ERR1378647.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378647.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273826
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA35000.27476280119890784No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA28740.22561951161304605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT3500.043.0671115
TCGGTAT4500.040.9958923
GCGGTCT4550.040.5453836
TTCGGTA4550.040.5453822
CGTCATA3800.040.2591224
ATTCGGT4650.039.6734421
CGGTATT4650.039.6734424
AGGCGGT4800.038.43364334
ACGTCAT4000.038.2461623
GACGTCA3950.038.1607322
TGACGTC3950.038.1607321
TAAGCCG3900.038.073113
TCGGGGA4850.038.03742214
GGCGGTC4850.038.03742235
CGGTCTC4850.038.03742237
CGGGGAA4950.037.26898615
AAGCCGA4150.036.8637714
ATGACGT4150.036.8637720
AATTCGG5050.036.53098720
GAATTCG5050.036.53098719
Produced by FastQC (version 0.11.5)