FastQCFastQC Report
Mon 6 Jun 2016
ERR1378646.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378646.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904150
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA42310.46795332632859593No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA32340.35768401260852734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT4650.041.12610215
TTCGGTA3700.040.7403422
GCCGATG4700.040.6885916
TAAGCCG4700.040.20990413
CGGTATT3750.040.19713624
CGATGAC4900.039.4869818
GAATTCG3850.039.15305319
TCGGTAT3850.039.15305323
CCGATGA5000.039.1472117
TGACGTC4950.039.0881221
CGGTCTC4000.038.80973437
GCGGTCT3950.038.73141536
GACGTCA5000.038.69724322
ACGTCAT5100.038.3796223
GGCGGTC4000.038.24727235
AATTCGG3950.038.1618420
CGTCATA5100.037.93847724
AAGCCGA5000.037.79730614
ATGACGT5200.037.6415520
GATGACG5200.037.6415519
Produced by FastQC (version 0.11.5)