FastQCFastQC Report
Mon 6 Jun 2016
ERR1378645.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378645.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094852
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA42990.39265581101372604No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA30340.2771150803944277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT5100.042.3503615
GATGACG5200.041.96859719
CCGATGA5200.041.5359317
ATGACGT5150.041.5023320
CGTCATA5150.041.5023324
CGATGAC5250.041.14035418
GCCGATG5300.040.7522416
TAAGCCG5350.040.37137213
ACGTCAT5300.040.32773623
GACGTCA5350.039.9508422
GCGGTCT4000.039.372636
TGACGTC5450.039.21779621
CGGTATT4050.038.8865224
TTCGGTA4100.038.41229622
AAGCCGA5700.038.28714414
AATTCGG4250.037.0565720
GAATTCG4200.036.96203619
GGCGGTC4350.036.20469335
TCGGTAT4350.036.20469323
CGGTCTC4400.035.79327437
Produced by FastQC (version 0.11.5)