FastQCFastQC Report
Mon 6 Jun 2016
ERR1378643.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378643.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164142
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA34830.29919030496279664No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA25860.222137849162731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA4000.042.18286524
TAAGCCG3900.042.11076413
AGCCGAT4150.041.20029415
GACGTCA4200.040.70981222
TGACGTC4150.040.65818821
CGATGAC4250.039.70152318
TTCGGTA2650.039.05231522
TCGGTAT2650.039.05231523
CCGATGA4350.038.7888517
ACGTCAT4450.038.4227523
GCCGATG4550.037.0838416
AAGCCGA4500.036.99593714
CGGTATT2800.036.9602324
GAATTCG2800.036.9602319
AATTCGG2850.036.31180220
ATGACGT4650.036.2863420
GATGACG4700.035.90031419
ATTCGGT3050.033.930721
GGCGGTC3150.032.85353535
GCGGTCT3100.032.65770736
Produced by FastQC (version 0.11.5)