FastQCFastQC Report
Mon 6 Jun 2016
ERR1378640.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378640.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593395
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA34650.5839280748910928No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA24520.41321548041355255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG3500.043.0705413
AGCCGAT3650.041.3005215
CGTCATA3750.040.1991724
ACGTCAT3850.039.15503723
CGGGGAA3850.038.57063315
TTCGGTA3850.038.57063322
GACGTCA3950.038.16377322
TCGGGGA3900.038.0761414
AGATTCG659.094947E-1238.0761410
TCGGTAT3900.038.0761423
TGACGTC4000.037.6867221
GCCGATG4000.037.6867216
AAGCCGA4000.037.6867214
CGATGAC4000.037.6867218
CGGTATT3950.037.59416624
CCGATGA4050.037.22145517
ATGACGT4150.036.32455420
ATTCGGT4100.036.21876521
AATTCGG4100.036.21876520
CTCGGGG3450.035.86882813
Produced by FastQC (version 0.11.5)