FastQCFastQC Report
Mon 6 Jun 2016
ERR1378633.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378633.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776160
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA32530.41911461554318696No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA24810.31965058750773034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG3600.043.1235813
GACGTCA3800.041.44600322
GCCGATG3800.041.44600316
CGATGAC3900.040.9601918
CCGATGA3850.040.90774517
TGACGTC3950.039.87210521
ATGACGT3950.039.87210520
CGTCATA3950.039.87210524
CGGTATT3600.039.37370724
GATGACG4000.039.37370319
AGCCGAT3950.039.302515
AGGCGGT3700.038.91763734
TTCGGTA3650.038.8343422
ACGTCAT4150.038.49270623
GAATTCG3800.038.48557719
AATTCGG3750.038.3987420
GCGGTCT3850.037.98576436
CGGTCTC3850.037.40136737
TCGGGGA3800.037.30140314
CGGGGAA3800.037.30140315
Produced by FastQC (version 0.11.5)