FastQCFastQC Report
Mon 6 Jun 2016
ERR1378632.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378632.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509388
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA26860.5272994259778401No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA18280.3588620069573684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG3700.042.56645213
CGTCATA3700.042.56645224
GACGTCA3850.040.90801622
ATGACGT3850.040.90801620
GATGACG3850.040.90801619
AGCCGAT3900.040.3835615
TGACGTC3900.040.3835621
ACGTCAT3900.040.3835623
CCGATGA3950.039.87237517
CGATGAC3950.039.87237518
AGATTCG750.038.9989810
AAGCCGA4050.038.8878714
GCGGTCT3050.038.3596536
GTCATAA4150.037.95081325
TTCGGTA3100.037.74094822
CGGTATT3150.037.14188424
TATGCCG850.037.05785443
GCCGATG4250.037.05785416
GAATTCG3150.036.42761619
AGGCGGT3250.035.99905834
Produced by FastQC (version 0.11.5)