FastQCFastQC Report
Mon 6 Jun 2016
ERR1378631.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378631.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647117
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA31280.48337472203635506No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA20780.3211165832453791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGTA3600.041.87190622
TCGGTAT3600.041.87190623
AATTCGG3650.041.2983220
GCGGTCT3600.041.2501436
CCGATGA3300.040.9092317
GCCGATG3300.040.9092316
CGATGAC3300.040.9092318
CGTCATA3300.040.9092324
GACGTCA3300.040.9060722
TCGGGGA3700.040.7433814
ATTCGGT3700.040.74023421
CGGTATT3650.040.68507424
AGCCGAT3350.040.2986515
TAAGCCG3350.040.2986513
CGGGGAA3800.039.6711915
GAATTCG3800.039.6711919
GGCGGTC3750.039.60013635
CTCGGGG3200.039.37513413
CCTCGGG3150.039.2828112
ACGTCAT3450.039.12754423
Produced by FastQC (version 0.11.5)