FastQCFastQC Report
Mon 6 Jun 2016
ERR1378629.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378629.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997081
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA31880.3197333015070992No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA24580.24651959068521012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT3600.041.24471315
GCCGATG3700.040.7380216
GACGTCA3600.039.99487322
TGACGTC3650.039.44721
CGATGAC3700.038.91393318
TAAGCCG3700.038.91393313
CGTCATA3650.038.8325924
TTCGGTA3150.038.56648322
CCGATGA3800.038.4819117
AATTCGG3250.038.07204420
GATTCGG659.094947E-1238.07204411
AAGCCGA3900.038.0720414
GATGACG3750.037.7951619
ACGTCAT3850.037.39780423
CGGTATT3250.037.38170224
ATTCGGT3350.036.93556621
GAATTCG3350.036.93556619
TCGGTAT3300.036.8134623
CGGGGAA3450.035.86496715
CGGTCTC3450.035.2146437
Produced by FastQC (version 0.11.5)