FastQCFastQC Report
Mon 6 Jun 2016
ERR1378624.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378624.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783735
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA37700.48102993996695315No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA28570.3645364823569191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCA3800.043.2217222
CGTCATA3800.043.2217224
TGACGTC3850.042.660421
ACGTCAT3850.042.660423
CCGATGA3900.042.11347617
TAAGCCG3950.041.5803913
GCCGATG4000.041.0606416
CGATGAC4050.040.55371518
TTCGGTA3900.040.38278622
CGGTATT3900.040.38278624
GATGACG4100.040.0591619
AATTCGG4000.039.37321520
GAATTCG4000.039.37321519
AGCCGAT4200.039.1053715
CCTCGGG3700.038.91715212
ATTCGGT4050.038.88712321
AAGCCGA4250.038.64530614
ATGACGT4250.038.64530620
GCGGTCT4200.038.5696836
TCGGTAT4100.038.4128923
Produced by FastQC (version 0.11.5)