FastQCFastQC Report
Mon 6 Jun 2016
ERR1378623.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378623.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966848
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA37280.3855828423909446No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA28080.2904282782815913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG3300.040.22380413
TCGGTAT3600.039.37160523
GACGTCA3400.039.04075222
ATTCGGT3650.038.83226821
CGGTATT3650.038.83226824
TGACGTC3500.038.56810421
TATGCCG700.038.56810443
AATTCGG3700.038.3075120
ACGTCAT3500.037.925323
GCGGTCT3750.037.7967436
GAATTCG3850.037.39937219
CGATGAC3550.037.39114418
CGTCATA3550.037.39114424
TCGGGGA3800.037.29941614
GATGACG3600.036.87182219
AGGCGGT3850.036.81500634
AGCCGAT3650.036.36672615
CGGGGAA3900.036.34301815
TTCGGTA3950.035.88298422
AAGCCGA3700.035.87528614
Produced by FastQC (version 0.11.5)