FastQCFastQC Report
Mon 6 Jun 2016
ERR1378622.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378622.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097375
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA37650.3430914682765691No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA26680.24312564073356874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAT4700.042.12510323
TAAGCCG4450.041.96565613
CGTCATA4450.041.96565624
GCGGTCT4650.041.6122736
GGCGGTC4700.041.16958635
CGGTATT4900.040.86672624
AATTCGG4850.040.8222620
TCGGGGA4800.040.7806314
CCGATGA4650.040.1606817
TTCGGTA4950.039.99756622
AGGCGGT4850.039.896334
ATTCGGT5050.039.20553621
TGACGTC4850.038.966721
AGCCGAT4800.038.90565515
CGATGAC4800.038.90565518
CGGGGAA5050.038.76178415
AAGCCGA4900.038.57084314
GAATTCG5150.038.44602219
CGGTCTC5250.038.14227737
TCCTCGG4350.037.7563311
Produced by FastQC (version 0.11.5)