FastQCFastQC Report
Mon 6 Jun 2016
ERR1378621.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378621.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808500
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA35950.4446505875077304No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA25670.31750154607297465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG800.042.18367443
GGCGGTC3400.041.02569235
ATTCGGT3350.040.96643421
AATTCGG3350.040.96643420
CGTCATA3750.040.79882424
CGGTATT3400.040.36648624
CGGGGAA3400.040.36398715
TTCGGTA3400.040.36398722
TCGGTAT3450.039.77923
TAAGCCG3850.039.73665213
ACGTCAT3900.039.2272123
AGGCGGT3500.039.21315434
GACGTCA3950.038.73066322
TGACGTC3950.038.73066321
ATGACGT3950.038.73066320
CGGTCTC3550.038.65846637
CCGATGA4000.038.2465317
GCCGATG4050.037.77435316
GCGGTCT3650.037.59932736
GAATTCG3650.037.59932719
Produced by FastQC (version 0.11.5)