FastQCFastQC Report
Mon 6 Jun 2016
ERR1378618.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378618.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775443
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA30610.3947421022563877No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA24030.30988738050378944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGA3900.040.9565514
CTCGGGG3300.040.2223713
AATTCGG4000.039.93263220
TCGGTAT4000.039.93263223
ACGTCAT3450.039.77776323
CGATGAC3450.039.77776318
CGGGGAA4050.039.43964415
TTCGGTA4050.039.43964422
CGTCATA3500.039.21203624
GATGACG3500.039.20950719
GAATTCG4150.039.0313919
GGCGGTC4100.038.9611835
ATTCGGT4100.038.95866821
TAAGCCG3600.038.7452813
ATGACGT3550.038.6572620
GCGGTCT4150.038.4917736
AGGCGGT4150.038.4917734
CGGTATT4150.038.4917724
GACGTCA3600.038.12035822
CAGGCGG4200.038.0335333
Produced by FastQC (version 0.11.5)