FastQCFastQC Report
Mon 6 Jun 2016
ERR1378616.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378616.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606663
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA28920.4767061778944818No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA20420.3365954409614564No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGACTAGATCTCGTATGCCGTC6240.10285776452495043Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTCT2950.044.23469536
TAAGCCG3700.043.78121613
TTCGGTA3050.043.5220422
ATTCGGT3150.042.8546321
TCGGTAT3100.042.82007223
CGATGAC3850.042.65983618
CCTCGGG2600.042.4013612
CGTCATA3850.042.07545524
AAGCCGA3950.041.5798414
CCGATGA3900.041.53602617
AATTCGG3250.041.53602620
CGGTATT3200.041.48194524
GGCGGTC3150.041.42614435
TGACGTC3950.041.01025421
GAATTCG3300.040.90669319
GATGACG4100.040.60737619
AGCCGAT4000.040.49762315
TCGGGGA3300.040.22491514
GACGTCA4050.039.99765822
GCCGATG4050.039.99765816
Produced by FastQC (version 0.11.5)