FastQCFastQC Report
Mon 6 Jun 2016
ERR1378614.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378614.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015981
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA31620.3112262926176769No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA22610.22254353181801628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGAT207.035969E-444.99601414
CGTCATA3750.041.99834424
ACGTCAT3800.041.44369523
ATGACGT3850.040.90546820
TAAGCCG3850.040.90546813
TCGGGGA3350.040.2949414
AATTCGG3350.040.2949420
GCCGATG4000.039.9339616
AGCCGAT3950.039.86988415
TCGGTAT3400.039.70236623
TTCGGTA3500.039.2108122
CCGATGA4100.038.95996517
GACGTCA4050.038.88544522
CGATGAC4150.038.49056618
GGCGGTC3450.038.47674635
TGACGTC4100.038.41123221
ATTCGGT3600.038.12162421
CGGGGAA3600.038.12162415
AGGCGGT3500.037.9270834
GCGGTCT3600.037.49852436
Produced by FastQC (version 0.11.5)