FastQCFastQC Report
Mon 6 Jun 2016
ERR1378613.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378613.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561806
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA33570.5975372281534908No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA22880.4072580214522451No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGACATCTCGTATGCCGTC6380.11356233290495295Illumina Paired End PCR Primer 2 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT4750.043.1017715
ATGACGT4850.042.21307420
CCGATGA4900.041.78232617
AAGCCGA4900.041.78232614
TAAGCCG4900.041.78232613
TCGGTAT2950.041.183123
CGATGAC5050.040.98677418
TGACGTC5000.040.94668221
GATGACG5000.040.94668219
CGTCATA5000.040.94668224
ACGTCAT5100.040.14380323
CGGTATT3100.039.91611524
GCGGTCT3050.039.83283236
GACGTCA5150.039.7540622
CTCGGGG2400.039.37180713
GAATTCG3150.039.28252819
TCGGGGA3100.039.19036514
GGCGGTC3100.039.19036535
TTCGGTA3100.039.19036522
GCCGATG5300.039.05343616
Produced by FastQC (version 0.11.5)