FastQCFastQC Report
Mon 6 Jun 2016
ERR1378612.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378612.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758530
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA27140.3577973184976204No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA19770.26063570326816343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGA2700.041.66428814
TCGGTAT2850.041.05028523
GCGGTCT2850.040.2635136
TTCGGTA2900.039.56670822
GGCGGTC2950.038.89864335
CCGATGA2550.038.82131217
TAAGCCG2550.038.82131213
CTCGGGG2300.038.14999813
TGACGTC2600.038.07474521
ACGTCAT2600.038.07474523
ATTCGGT3050.037.620821
AATTCGG3050.037.620820
CGTCATA2650.037.35881824
GACGTCA2650.037.35635422
CGGTATT3150.037.14318524
AAGCCGA2750.035.99794414
GAATTCG3200.035.8596919
CGGGGAA3150.035.71224615
TATGCCG1200.035.62296343
GCCGATG2800.035.3551216
Produced by FastQC (version 0.11.5)