FastQCFastQC Report
Mon 6 Jun 2016
ERR1378611.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378611.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497302
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA35870.7212920921291287No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA24790.4984898512372764No Hit
TCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA5200.10456422857740366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATGA3850.042.66081217
TAAGCCG3850.042.66081213
GACGTCA3950.041.58079522
CGTCATA3950.041.58079524
ACGTCAT4000.041.0610323
ATGACGT4050.040.55410820
CGATGAC4050.040.55410818
AGCCGAT4100.040.05954415
TGACGTC4100.040.05954421
GATGACG4100.040.05954419
AGATTCG451.9252184E-839.99857310
TCGGGGA3450.039.12903614
GCCGATG4200.039.10574316
AAGCCGA4200.039.10574314
ATTCGGT3500.038.5700521
AATTCGG3500.038.5700520
TTCGGTA3550.038.0268122
TCGGTAT3550.038.0268123
GCGGTCT3500.037.92721636
GAATTCG3600.037.4986619
Produced by FastQC (version 0.11.5)