FastQCFastQC Report
Mon 6 Jun 2016
ERR1378608.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378608.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918426
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA40950.4458715236720215No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA28840.3140155004322613No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAGATATCTCGTATGCCGTC10980.11955236458898158Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG4300.042.37951713
CGTCATA4300.041.8563124
ACGTCAT4600.039.12655323
GACGTCA4750.037.8909822
CGGTATT4350.037.75487524
TCGGTAT4400.037.32584423
TGACGTC4850.037.10972221
ATTCGGT4450.036.9064521
CCGATGA4850.036.6458517
TCGGGGA4550.036.5897814
TTCGGTA4500.036.4963822
ATGACGT4900.036.27190820
CGATGAC5000.035.9964318
GCGGTCT4650.035.80290236
AGCCGAT5100.035.2906215
GATGACG5200.035.044619
GAATTCG4700.034.9433419
CCTCGGG3950.034.7433912
CTCGGGG3950.034.7433913
AAGCCGA5250.034.71084214
Produced by FastQC (version 0.11.5)