FastQCFastQC Report
Mon 6 Jun 2016
ERR1378606.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378606.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1039286
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA35730.3437937199192523No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA26320.253250789484319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG3850.043.2443513
CGTCATA3850.042.6640724
CGGTATT3900.040.96320324
TCGGTAT3900.040.9612323
ATGACGT4100.040.6094520
AGCCGAT4150.040.1182515
TTCGGTA4050.039.4441522
ACGTCAT4250.039.17617823
GCGGTCT4150.038.49368736
GACGTCA4400.038.35198622
ATTCGGT4250.038.11736321
CCGATGA4400.037.83880617
GCCGATG4400.037.83880616
GAATTCG4350.037.241119
TGACGTC4550.037.08763521
CGATGAC4600.036.68450518
TCGGGGA4300.036.6258714
GATGACG4600.036.1953819
AAGCCGA4600.036.1936414
AGGCGGT4400.035.7969134
Produced by FastQC (version 0.11.5)