FastQCFastQC Report
Mon 6 Jun 2016
ERR1378604.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378604.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773356
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA34170.4418404977785134No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA26520.342920983350488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA3800.042.03832624
GCGGTCT4200.041.78457636
AGCCGAT3850.040.90797415
TAAGCCG3850.040.90797413
CCGATGA4000.039.9364117
CGGTATT4400.039.88527724
TCGGTAT4400.039.88527723
TCGGGGA4450.039.43712214
ATTCGGT4450.039.43712221
CGGGGAA4450.039.43712215
GAATTCG4450.039.43712219
GACGTCA4000.039.37392422
ATGACGT4000.039.37392420
AGGCGGT4500.038.99893634
CGGTCTC4500.038.99893637
ACGTCAT4050.038.88782523
GGCGGTC4550.038.57037435
GCCGATG4200.038.03467616
GATGACG4150.037.9507719
TTCCTCG3750.037.7989710
Produced by FastQC (version 0.11.5)