FastQCFastQC Report
Mon 6 Jun 2016
ERR1378602.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378602.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782200
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA35800.4576834569163897No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA29010.3708770135515213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGG4150.042.28783420
GCGGTCT4150.041.7456836
TTCGGTA4150.041.7456822
TAAGCCG4000.041.0612213
ATTCGGT4250.040.7634321
TCGGTAT4250.040.7634323
CCGATGA4050.040.5542917
GGCGGTC4350.039.82634435
GAATTCG4350.039.82634419
CGTCATA4100.039.5109624
AGGCGGT4400.039.3737734
AGCCGAT4200.039.10592315
ACGTCAT4150.039.03492423
CGGTATT4450.038.93136624
CGATGAC4250.038.6458518
TGACGTC4300.038.1964821
ATGACGT4250.038.11645520
CGGTCTC4550.038.07573337
CTCGGGG3800.037.8935513
GACGTCA4300.037.6732422
Produced by FastQC (version 0.11.5)