FastQCFastQC Report
Mon 6 Jun 2016
ERR1378601.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378601.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712012
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA29770.41811093071465083No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA25260.3547693016409836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG3750.041.39689613
CGTCATA3900.040.3815924
ATTCGGT2600.038.9393921
AGCCGAT4050.038.8859815
TCGGTAT2550.038.8206223
ATGACGT4100.038.41175520
GCGGTCT2650.038.2046836
CCGATGA4150.037.94896317
CGATGAC4150.037.94896318
TGACGTC4250.037.58542321
TTCGGTA2700.037.4971922
GACGTCA4250.037.0560522
AGGCGGT2750.036.81542634
AATTCGG2750.036.81542620
AAGCCGA4300.036.62516414
GATGACG4300.036.62516419
GGCGGTC2800.036.15800535
CGGTCTC2800.036.15800537
CGGTATT2750.035.99730324
CTCGGGG2450.035.81364413
Produced by FastQC (version 0.11.5)