FastQCFastQC Report
Mon 6 Jun 2016
ERR1378600.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378600.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687132
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA44070.6413614851294948No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA33720.4907354045510906No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTATGATCTCGTATGCCGTC8250.12006426712771345Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG5550.041.3499513
CGTCATA5850.039.99864224
CCGATGA5800.039.9555417
ATGACGT5850.039.61403720
TGACGTC5900.039.27832421
CGATGAC5900.039.27832418
AGCCGAT5900.038.89698415
ACGTCAT6000.038.6236923
GACGTCA6100.037.9905122
GATGACG6100.037.9905119
TATAAGC6050.037.932611
CGGTATT5000.037.79871724
AAGCCGA6200.037.01487414
TCGGTAT5000.036.89874623
TCGGGGA5100.036.616414
GACCCTT6350.036.49482339
TCATAAC6300.036.42733426
TTCGGTA5100.036.17524322
GGTATCG250.002106871535.99877513
GGATTTA6450.035.5853656
Produced by FastQC (version 0.11.5)