FastQCFastQC Report
Mon 6 Jun 2016
ERR1378599.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378599.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871202
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA30290.34768056088025506No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA25870.2969460584342093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG3750.042.59755313
AGCCGAT3950.040.44071615
CGTCATA3950.040.44071624
TCGGTAT3300.040.22496823
GACGTCA4100.039.5099322
AGGCGGT3250.039.45927434
TGACGTC4050.039.4421821
ATGACGT4050.039.4421820
GCGGTCT3350.038.95298836
GGCGGTC3300.038.8614135
AATTCGG3500.038.56921820
TTCGGTA3500.038.56921822
GATTCGG700.038.56921411
GCCGATG4150.038.49176416
ACGTCAT4150.038.49176423
CGGTATT3450.038.47605524
AAGCCGA4200.038.0335314
CGGTCTC3650.036.9841837
GAATTCG3600.036.87288319
TCGGGGA3550.036.75845314
Produced by FastQC (version 0.11.5)