FastQCFastQC Report
Mon 6 Jun 2016
ERR1378598.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378598.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672299
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA24840.36947846122037964No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA20570.3059650542392596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG2800.041.78205513
TGACGTC2900.041.1170921
CCGATGA2850.041.04903417
TCGGGGA2650.040.75114414
TTCCTCG2500.040.49645210
TTCGGTA2700.039.996522
TCGGTAT2700.039.996523
ATGACGT3000.039.7465220
AGCCGAT2950.039.65754315
AAGCCGA2950.039.65754314
ATTCGGT2750.039.26928321
GGCGGTC2750.039.26928335
CGGTATT2750.039.26928324
AGGCGGT2850.038.6808234
AATTCGG2850.038.6808220
ACGTCAT3050.038.35729223
CGTCATA3050.038.35729224
GACGTCA3100.037.7386322
CTCGGGG2700.037.49671613
CGGGGAA2900.037.23811715
Produced by FastQC (version 0.11.5)