FastQCFastQC Report
Mon 6 Jun 2016
ERR1378597.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378597.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898553
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA27410.30504600173835045No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA21380.23793810715672867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG3000.040.49699813
TTCGGTA2250.039.9970322
TCGGTAT2250.039.9970323
CGATGAC3200.039.37207818
CGGTATT2300.039.1275324
AATTCGG2350.038.29503320
CGTCATA3250.037.38184424
CCGATGA3350.036.9375617
GGCGGTC2500.036.8993235
CGGGGAA2500.036.89726315
AGGCGGT2450.036.7340134
ATTCGGT2450.036.73196821
ACGTCAT3350.036.2659723
GCGGTCT2550.035.29346536
AGCCGAT3450.035.2147815
GGTATTC2650.034.8087425
TGACGTC3500.034.7117121
AAGCCGA3500.034.7117114
ATGACGT3500.034.7117120
GACGTCA3550.034.22281322
Produced by FastQC (version 0.11.5)