FastQCFastQC Report
Mon 6 Jun 2016
ERR1378596.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378596.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681320
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA22180.3255445312041332No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA18020.26448658486467447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG2850.042.63067213
ATTCGGT2850.041.05175821
TTCGGTA2800.040.98127422
TCGGTAT2800.040.98127423
TGACGTC3050.039.8352221
GCCGATG3050.039.8352216
TCGGGGA3000.039.74915714
CGGTATT2950.039.6601824
TCCTCGG2450.039.48895611
CCGATGA3100.039.19271517
AAGCCGA3100.039.19271514
CGTCATA3100.039.19271524
AGCCGAT3150.038.5706115
ATGACGT3150.038.5706120
ACGTCAT3150.038.5706123
CGATGAC3150.038.5706118
GGCGGTC3100.038.46692735
AATTCGG3000.038.24918720
GAATTCG3100.037.74113519
GACGTCA3250.037.38382322
Produced by FastQC (version 0.11.5)